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- Mitochondrial dynamics quantitatively revealed by STED . . . - Nature
Here, we developed an enhanced squaraine variant dye (MitoESq-635) to study the dynamic structures of mitochondrial cristae in live cells with a superresolution technique
- Protocol for assessing real-time changes in mitochondrial morphology . . .
Changes in mitochondrial size, shape, and subcellular position, a process collectively known as mitochondrial dynamics, are exploited for various cancer traits
- Confocal Microscopy of Mitochondrial Function in Living Cells
Confocal microscopy is an essential tool for studying the physiology and pathophysiology of mitochondria within single living cells As more parameter-indicating fluorophores are discovered, the usefulness of confocal microscopy will only increase
- Spinning Disk Confocal Microscopy for Optimized and Quantified Live . . .
In contrast, confocal microscopy does not only allow for assessing the mitochondrial ultra-structure, but it has sufficient resolution for studying the 3D mitochondrial network in live cells, with the possibility of assessing the mitochondrial membrane potential
- Understanding Mitochondrial and Lysosomal Dynamics by . . . - Springer
Live cell imaging is a robust method to visualize dynamic cellular structures, especially organelles with network-like structures such as mitochondria In this regard, mitochondrial dynamics, namely mitochondrial fission and fusion, are highly dynamic processes that
- Live-cell imaging powered by computation - Nature
We specifically discuss how computational approaches can improve the signal-to-noise ratio, spatial resolution, temporal resolution and multi-colour capacity of live-cell imaging An
- Live-cell STED nanoscopy of mitochondrial cristae - Nature
Using live-cell STED nanoscopy, we were able to record entire cells, thereby getting an overview on the cristae architecture of mitochondria with different shapes or with different
- Protocol for live-cell imaging of mitochondrial dynamics in adult . . .
Mitochondrial dynamics are essential for cellular homeostasis and can be visualized in adult Drosophila oenocytes using live-cell confocal imaging Here, we present a protocol for live-cell imaging of mitochondrial dynamics in adult Drosophila oenocytes
- Multispectral live-cell imaging with uncompromised . . . - Nature
In this Article, we present an optimized approach for simultaneous multispectral acquisition and subsequent offline unmixing of fluorescence microscopy data that is ideally suited for imaging
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